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Organic Analytical Analyses: Lab Hacks and Best Practices - The Science of Real Life

AVAILABLE IN: English

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Presented by:
• Patricia Atkins – Senior Applications Scientist Spex, an Antylia Scientific company

In this webinar you will:

  • Understand the function and interaction of common organic analysis techniques such as spectroscopy and chromatography
  • Review best practices for common laboratory sample preparation and dilution techniques used in organic sample preparation to reduce contamination and error
  • Learn about laboratory hacks and practices to help you streamline and improve your sample preparation, standard use, and sample analysis

Chromatography and spectroscopy are the most common analytical techniques in the organic laboratory. By understanding the operation and chemistry involved in these processes, you can optimize your analyses and eliminate errors. This presentation will explore ways to improve organic analyses with information regarding best practices and lab hacks to aid in the organic laboratory.

Presenters

Your presenters for this webinar

Patricia Atkins – Senior Applications Scientist Spex, an Antylia Scientific company  
Patricia Atkins is a Senior Applications Scientist. She is a graduate of Rutgers University in NJ and was laboratory supervisor for Ciba Specialty Chemicals in the Water Treatment Division. Patricia later accepted a position conducting research and managing an air pollution research group within Rutgers University’s Civil & Environmental Engineering Department. In 2008, Patricia joined Spex as a senior application scientist in our certified reference materials’ division and spends her time researching industry trends and developing new reference materials. Patricia has been involved with many industry-focused advisory and regulatory groups including AOAC, ASTM, ACIL, NACRW and the Emerald Conference. She is a frequent presenter and speaker at numerous conferences including NACRW, NEMC, Pittcon and AOAC. She is a published author with her work appearing in various journals and trade publications including Spectroscopy, LCGC and Cannabis Science and Technology where she is a columnist for analytical issues in cannabis testing.


Questions & Answers from the live event

There are now solvents designated as LCMS solvents not just HPLC solvents; is there really a difference?

The LCMS solvents are more highly filtered and characterized for ionic contamination since it could affect LCMS. In most cases with analytes in the ppm range or high ppb range, the HPLC solvents are sufficient. In delicate and precise analyses in the ppb or lower range or in cases where the background noise is hindering quantitation, then it might be worth investing in the extra cost for LCMS solvents.

Should my lab prefilter my mobile phases prior to use or are the filters in the system enough?

During manufacturing, most LC and LCMS solvents are filtered. If you are using good-quality solvents, then filtering may be unnecessary. If you add any buffers, especially solid materials to the mobile phase, then the mobile phases should be filtered.

Is there are mix of solvents which is best for flushing out an HPLC system?

It is safe to use most LC columns with many types of solvents but check with the manufacturer's documentation. Generally, a mix of polar and nonpolar solvents with a wide range of functionalities can be a good flush for an HPLC system. Some popular solvent mixes contain IPA, MeOH, ACN, DCM, and acetone to flush systems.

Any tips on how to appropriately choose buffer strength?

The rule of thumb for adding buffers is to use the least amount necessary to get the result desired. Start low and then add up for the best result. Most buffers are added in the low molar or mini-molar range. You can also calculate the ion strength and dissociation which allows for the ion pairing action in some buffers.

Where can I get a copy of the method validation requirements?

Validation requirements differ depending on the quality management system of standards followed. The validation requirements can vary between ISO, ASTM, AOAC, etc. Consult your quality management system and standards organizations for a detailed requirement procedure.

With pre-refrigerated glassware and reagents, how serious is the risk of error due to moisture condensation?

There is always the possibility for moisture condensation with changes in temperature. Best practice is to keep all flasks closed when not in use for weighing. It also depends on the type of volatile compounds analyzed. You may be able to purge the glassware after measurement to keep moist air out of the vessel.



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